| B. Common histologic stains |
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| H&E | Hematoxylin and eosin. Cytoplasm is pink and nuclei are blue. Used routinely for light microscopy. |
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| GMS | Gomorri Methenamine Silver. Stains fungi including pneumocystis, black. Counterstain is green. |
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| AFB | Acid Fast Bacilli (Ziehl-Neelson) AFB are stained pink/red. Counterstain is blue. |
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| Fite | Modified AFB stain. Acid is dilute so weakly acid fast organisms eg atypical mycobacteria can be stained - colors are same as in AFB stain.
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| Auramine-Rhodamine | Fluorescent dye visualized under ultraviolet light. Rapid screening, usually used for smears. |
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| EVG | Elastic Van Gieson. Stains elastic tissue black. Counterstain varies from yellow to pink. |
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| Trichrome | Masson trichrome most commonly used. Stains collagen (fibrous tissue) blue and muscle red. |
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| Immunoperoxidase | "Brown stain." Section of tissue on glass slide is layered with primary antibody which may be monoclonal or polyclonal. Antigen-antibody reaction is then detected by peroxidase-labeled antibody. The color reaction is brown. Occasionally a red color reaction is used. Counterstain is hematoxylin (blue). The specificity lies not in the color but in the primary antibody used.
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| Angiocentric | References |
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| Angiocentric | References |
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