<h1>Microbiology & Immunology - Extracting DNA from Bananas</h1>
 
Extracting DNA from Bananas
 

Ana Shulla, Oana Ciupuliga & Mark Clementz

Presented to:

Ms. Linda Salazar Seventh Grade Class
Annunciation B.V.M. School
1840 Church Rd., Aurora IL 60505
November 6, 2007

  

   
  1. Using a plastic fork, crush one banana thoroughly and mix it with ½ cup of water in a beaker
     

  2. Mix 1 teaspoon of soap with ¼ teaspoon of salt in a plastic cup. Add 2 tablespoons of water. Stir gently to avoid creating foam. Continue for a few minutes until the soap and salt are dissolved.
     

  3. Add 2 tablespoons of the banana mixture to the cup containing the soap solution. Use a spoon to stir the mixture for at least 10 minutes.

  4. Insert a filter into a clean plastic cup so it does not touch the bottom of the cup. 
      

  5. Pour the mixture from step 3 into the filter. After 10 minutes, some liquid, called the filtrate, should have collected in the bottom of the cup. Remove the filter and set it aside.
     

  6. Get a test tube of cold alcohol. Use a pipette to collect the filtrate and add it to the alcohol.
     

  7. Let the test tube with the alcohol and filtrate sit undisturbed for a few minutes. The white material coming out of solution as a precipitate is DNA.
      

  8. Dip the applicator stick into the tube, slowly rotating to spool out the banana's DNA.

Ms. Linda Salazar Seventh Grade Class

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