| Imaging
The core facility has four systems available for imaging purposes: theChemi-doc, the Odyssey, the ProXpress, and the phosphoimager.
The ChemiDoc system combines gel documentation technology with the enhanced chemiluminescent detection capabilities of a supercooled camera.
The Odyssey features two-color, infrared detection for simultaneous detection of two anitbodies; the sensitivity is equal to or better than chemiluminescence on standard Western blots. Wide linear range and direct fluorescence detection give Odyssey high quantification accuracy.
The ProXPRESS™ Proteomic Imaging System enables the use of all dyes in the UV and VIS spectrum range with its CCD-based detection and multi-wavelength emission and excitation capabilities.
The Packard “Cyclone” phosphorimager performs filmless autoradiography of electrophoresis gels, blots, thin-layer chromatography samples, tissue sections, and cDNA microarrays. Reusable storage phosphor screens capture and store the activity of samples, which are exposed in common film cassettes. The screens are then scanned by a laser focused to less than 50 microns, and the latent image is detected by confocal optics to create a high-resolution digitized image with quantitative data. The phosphorimaging station can be used to capture various types of images and data obtained from several types of experiments, including Northerns, Westerns, Southerns, cDNA microarrays, and in situ hybridizations. |